WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
ATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAACCATGAAACCAACATAAACATTAT

Read1 before filtering:
total reads: 5095450
total bases: 733744800
Q20 bases: 711938865(97.0281%)
Q30 bases: 682558405(93.024%)

Read1 after filtering:
total reads: 5055266
total bases: 726538976
Q20 bases: 706391963(97.227%)
Q30 bases: 677561107(93.2587%)

Filtering result:
reads passed filter: 5055266
reads failed due to low quality: 14761
reads failed due to too many N: 6990
reads failed due to too short: 18433
reads with adapter trimmed: 45407
bases trimmed due to adapters: 4069206

Duplication rate (may be overestimated since this is SE data): 78.9596%

JSON report: SewardAK_Q316.json
HTML report: SewardAK_Q316.html

fastp -i SewardAK_Q316.F.fq.gz -o SewardAK_Q316.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q316.json -h SewardAK_Q316.html 
fastp v0.20.0, time used: 50 seconds