WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2996762
total bases: 431533728
Q20 bases: 418541676(96.9893%)
Q30 bases: 401135540(92.9558%)

Read1 after filtering:
total reads: 2984107
total bases: 429708348
Q20 bases: 417622334(97.1874%)
Q30 bases: 400436586(93.188%)

Filtering result:
reads passed filter: 2984107
reads failed due to low quality: 8563
reads failed due to too many N: 4092
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 72.2837%

JSON report: SewardAK_Q314.json
HTML report: SewardAK_Q314.html

fastp -i SewardAK_Q314.F.fq.gz -o SewardAK_Q314.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q314.json -h SewardAK_Q314.html 
fastp v0.20.0, time used: 37 seconds