WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAGC

Read1 before filtering:
total reads: 3631000
total bases: 522864000
Q20 bases: 508288070(97.2123%)
Q30 bases: 487966199(93.3256%)

Read1 after filtering:
total reads: 3610820
total bases: 519607932
Q20 bases: 505613766(97.3068%)
Q30 bases: 485576956(93.4506%)

Filtering result:
reads passed filter: 3610820
reads failed due to low quality: 6020
reads failed due to too many N: 2130
reads failed due to too short: 12030
reads with adapter trimmed: 24127
bases trimmed due to adapters: 2080867

Duplication rate (may be overestimated since this is SE data): 70.2832%

JSON report: SewardAK_Q307.json
HTML report: SewardAK_Q307.html

fastp -i SewardAK_Q307.F.fq.gz -o SewardAK_Q307.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q307.json -h SewardAK_Q307.html 
fastp v0.20.0, time used: 34 seconds