WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AAATGTTTTTGAGATGGCAGCAACGGAAACCATAACGAGCATCATCTTGATTAAGCTCAT

Read1 before filtering:
total reads: 2677373
total bases: 385541712
Q20 bases: 374644936(97.1736%)
Q30 bases: 359674543(93.2907%)

Read1 after filtering:
total reads: 2657508
total bases: 382569787
Q20 bases: 372126504(97.2702%)
Q30 bases: 357371839(93.4135%)

Filtering result:
reads passed filter: 2657508
reads failed due to low quality: 4753
reads failed due to too many N: 1606
reads failed due to too short: 13506
reads with adapter trimmed: 29805
bases trimmed due to adapters: 2054827

Duplication rate (may be overestimated since this is SE data): 75.4214%

JSON report: SewardAK_Q303.json
HTML report: SewardAK_Q303.html

fastp -i SewardAK_Q303.F.fq.gz -o SewardAK_Q303.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q303.json -h SewardAK_Q303.html 
fastp v0.20.0, time used: 26 seconds