WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4979536
total bases: 717053184
Q20 bases: 694101675(96.7992%)
Q30 bases: 651199964(90.8161%)

Read1 after filtering:
total reads: 4975954
total bases: 715938562
Q20 bases: 693670563(96.8897%)
Q30 bases: 650967992(90.9251%)

Filtering result:
reads passed filter: 4975954
reads failed due to low quality: 2121
reads failed due to too many N: 1461
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 44.8663%

JSON report: SewardAK_320.json
HTML report: SewardAK_320.html

fastp -i SewardAK_320.F.fq.gz -o SewardAK_320.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_320.json -h SewardAK_320.html 
fastp v0.20.0, time used: 56 seconds