WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2375194
total bases: 342027936
Q20 bases: 331100076(96.805%)
Q30 bases: 311004450(90.9295%)

Read1 after filtering:
total reads: 2373470
total bases: 341504332
Q20 bases: 330896745(96.8939%)
Q30 bases: 310895203(91.037%)

Filtering result:
reads passed filter: 2373470
reads failed due to low quality: 1042
reads failed due to too many N: 682
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 40.9817%

JSON report: SewardAK_315.json
HTML report: SewardAK_315.html

fastp -i SewardAK_315.F.fq.gz -o SewardAK_315.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_315.json -h SewardAK_315.html 
fastp v0.20.0, time used: 33 seconds