WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3342476
total bases: 481316544
Q20 bases: 464884917(96.5861%)
Q30 bases: 434965055(90.3699%)

Read1 after filtering:
total reads: 3339499
total bases: 480733451
Q20 bases: 464605655(96.6452%)
Q30 bases: 434790044(90.4431%)

Filtering result:
reads passed filter: 3339499
reads failed due to low quality: 1984
reads failed due to too many N: 993
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 40.23%

JSON report: SewardAK_312.json
HTML report: SewardAK_312.html

fastp -i SewardAK_312.F.fq.gz -o SewardAK_312.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_312.json -h SewardAK_312.html 
fastp v0.20.0, time used: 46 seconds