WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2330390
total bases: 335576160
Q20 bases: 325011519(96.8518%)
Q30 bases: 305203129(90.949%)

Read1 after filtering:
total reads: 2328794
total bases: 335030221
Q20 bases: 324807504(96.9487%)
Q30 bases: 305096191(91.0653%)

Filtering result:
reads passed filter: 2328794
reads failed due to low quality: 898
reads failed due to too many N: 698
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 42.079%

JSON report: SewardAK_311.json
HTML report: SewardAK_311.html

fastp -i SewardAK_311.F.fq.gz -o SewardAK_311.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_311.json -h SewardAK_311.html 
fastp v0.20.0, time used: 31 seconds