WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2794957
total bases: 402473808
Q20 bases: 389644169(96.8123%)
Q30 bases: 365750209(90.8755%)

Read1 after filtering:
total reads: 2793032
total bases: 402027915
Q20 bases: 389442533(96.8695%)
Q30 bases: 365625329(90.9453%)

Filtering result:
reads passed filter: 2793032
reads failed due to low quality: 1129
reads failed due to too many N: 796
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 41.3635%

JSON report: SewardAK_310.json
HTML report: SewardAK_310.html

fastp -i SewardAK_310.F.fq.gz -o SewardAK_310.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_310.json -h SewardAK_310.html 
fastp v0.20.0, time used: 37 seconds