WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3126512
total bases: 450217728
Q20 bases: 435473922(96.7252%)
Q30 bases: 408508842(90.7358%)

Read1 after filtering:
total reads: 3124316
total bases: 449712064
Q20 bases: 435249098(96.7839%)
Q30 bases: 408373475(90.8078%)

Filtering result:
reads passed filter: 3124316
reads failed due to low quality: 1371
reads failed due to too many N: 825
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 38.0951%

JSON report: SewardAK_309.json
HTML report: SewardAK_309.html

fastp -i SewardAK_309.F.fq.gz -o SewardAK_309.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_309.json -h SewardAK_309.html 
fastp v0.20.0, time used: 40 seconds