WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3305146
total bases: 475941024
Q20 bases: 460737357(96.8056%)
Q30 bases: 432339108(90.8388%)

Read1 after filtering:
total reads: 3302927
total bases: 475438476
Q20 bases: 460506803(96.8594%)
Q30 bases: 432194785(90.9045%)

Filtering result:
reads passed filter: 3302927
reads failed due to low quality: 1312
reads failed due to too many N: 907
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 39.8242%

JSON report: SewardAK_308.json
HTML report: SewardAK_308.html

fastp -i SewardAK_308.F.fq.gz -o SewardAK_308.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_308.json -h SewardAK_308.html 
fastp v0.20.0, time used: 41 seconds