WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3647077 total bases: 525179088 Q20 bases: 507365043(96.608%) Q30 bases: 476174015(90.6689%) Read1 after filtering: total reads: 3644512 total bases: 524385487 Q20 bases: 507056321(96.6953%) Q30 bases: 476005646(90.774%) Filtering result: reads passed filter: 3644512 reads failed due to low quality: 1484 reads failed due to too many N: 1081 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 40.7685% JSON report: SewardAK_306.json HTML report: SewardAK_306.html fastp -i SewardAK_306.F.fq.gz -o SewardAK_306.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_306.json -h SewardAK_306.html fastp v0.20.0, time used: 44 seconds