WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 2359698 total bases: 339796512 Q20 bases: 328790435(96.761%) Q30 bases: 308743118(90.8612%) Read1 after filtering: total reads: 2358057 total bases: 339262887 Q20 bases: 328585512(96.8528%) Q30 bases: 308632609(90.9715%) Filtering result: reads passed filter: 2358057 reads failed due to low quality: 924 reads failed due to too many N: 717 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 43.7053% JSON report: SewardAK_305.json HTML report: SewardAK_305.html fastp -i SewardAK_305.F.fq.gz -o SewardAK_305.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_305.json -h SewardAK_305.html fastp v0.20.0, time used: 33 seconds