WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2184407
total bases: 314554608
Q20 bases: 304210613(96.7115%)
Q30 bases: 285180403(90.6617%)

Read1 after filtering:
total reads: 2182631
total bases: 314173050
Q20 bases: 304034776(96.773%)
Q30 bases: 285072263(90.7373%)

Filtering result:
reads passed filter: 2182631
reads failed due to low quality: 1120
reads failed due to too many N: 656
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 41.8984%

JSON report: SewardAK_304.json
HTML report: SewardAK_304.html

fastp -i SewardAK_304.F.fq.gz -o SewardAK_304.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_304.json -h SewardAK_304.html 
fastp v0.20.0, time used: 31 seconds