WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3467160 total bases: 499271040 Q20 bases: 483272500(96.7956%) Q30 bases: 453608437(90.8541%) Read1 after filtering: total reads: 3464734 total bases: 498474869 Q20 bases: 482971557(96.8899%) Q30 bases: 453449335(90.9673%) Filtering result: reads passed filter: 3464734 reads failed due to low quality: 1431 reads failed due to too many N: 995 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 44.5307% JSON report: SewardAK_302.json HTML report: SewardAK_302.html fastp -i SewardAK_302.F.fq.gz -o SewardAK_302.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_302.json -h SewardAK_302.html fastp v0.20.0, time used: 43 seconds