WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1456933
total bases: 209798352
Q20 bases: 202884067(96.7043%)
Q30 bases: 190096661(90.6092%)

Read1 after filtering:
total reads: 1455717
total bases: 209545303
Q20 bases: 202765990(96.7648%)
Q30 bases: 190022777(90.6834%)

Filtering result:
reads passed filter: 1455717
reads failed due to low quality: 775
reads failed due to too many N: 441
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 47.1218%

JSON report: SewardAK_301.json
HTML report: SewardAK_301.html

fastp -i SewardAK_301.F.fq.gz -o SewardAK_301.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_301.json -h SewardAK_301.html 
fastp v0.20.0, time used: 24 seconds