WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2873252
total bases: 413748288
Q20 bases: 400026994(96.6837%)
Q30 bases: 375218757(90.6877%)

Read1 after filtering:
total reads: 2871110
total bases: 413167933
Q20 bases: 399792210(96.7626%)
Q30 bases: 375088212(90.7835%)

Filtering result:
reads passed filter: 2871110
reads failed due to low quality: 1358
reads failed due to too many N: 784
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 42.5626%

JSON report: SewardAK_300.json
HTML report: SewardAK_300.html

fastp -i SewardAK_300.F.fq.gz -o SewardAK_300.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_300.json -h SewardAK_300.html 
fastp v0.20.0, time used: 40 seconds