WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
CTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAA

Read1 before filtering:
total reads: 3872032
total bases: 557572608
Q20 bases: 541345404(97.0897%)
Q30 bases: 519124649(93.1044%)

Read1 after filtering:
total reads: 3840901
total bases: 553040323
Q20 bases: 537923917(97.2667%)
Q30 bases: 516040500(93.3097%)

Filtering result:
reads passed filter: 3840901
reads failed due to low quality: 9750
reads failed due to too many N: 5106
reads failed due to too short: 16275
reads with adapter trimmed: 22529
bases trimmed due to adapters: 2388848

Duplication rate (may be overestimated since this is SE data): 66.6139%

JSON report: SekiuWA_FG033.json
HTML report: SekiuWA_FG033.html

fastp -i SekiuWA_FG033.F.fq.gz -o SekiuWA_FG033.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG033.json -h SekiuWA_FG033.html 
fastp v0.20.0, time used: 37 seconds