WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3620548
total bases: 521358912
Q20 bases: 503398668(96.5551%)
Q30 bases: 472472318(90.6232%)

Read1 after filtering:
total reads: 3617920
total bases: 520535217
Q20 bases: 503081668(96.647%)
Q30 bases: 472302725(90.7341%)

Filtering result:
reads passed filter: 3617920
reads failed due to low quality: 1565
reads failed due to too many N: 1063
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 45.0904%

JSON report: SekiuWA_FG022.json
HTML report: SekiuWA_FG022.html

fastp -i SekiuWA_FG022.F.fq.gz -o SekiuWA_FG022.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG022.json -h SekiuWA_FG022.html 
fastp v0.20.0, time used: 45 seconds