WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2198953
total bases: 316649232
Q20 bases: 307137693(96.9962%)
Q30 bases: 288643296(91.1555%)

Read1 after filtering:
total reads: 2197431
total bases: 316344000
Q20 bases: 306985047(97.0415%)
Q30 bases: 288541491(91.2113%)

Filtering result:
reads passed filter: 2197431
reads failed due to low quality: 866
reads failed due to too many N: 656
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 47.0346%

JSON report: SekiuWA_FG020.json
HTML report: SekiuWA_FG020.html

fastp -i SekiuWA_FG020.F.fq.gz -o SekiuWA_FG020.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG020.json -h SekiuWA_FG020.html 
fastp v0.20.0, time used: 31 seconds