WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACG

Read1 before filtering:
total reads: 2811546
total bases: 404862624
Q20 bases: 392842652(97.0311%)
Q30 bases: 376553958(93.0078%)

Read1 after filtering:
total reads: 2776928
total bases: 399837133
Q20 bases: 388679081(97.2094%)
Q30 bases: 372700279(93.213%)

Filtering result:
reads passed filter: 2776928
reads failed due to low quality: 7420
reads failed due to too many N: 3636
reads failed due to too short: 23562
reads with adapter trimmed: 29764
bases trimmed due to adapters: 3430992

Duplication rate (may be overestimated since this is SE data): 70.192%

JSON report: SekiuWA_FG019.json
HTML report: SekiuWA_FG019.html

fastp -i SekiuWA_FG019.F.fq.gz -o SekiuWA_FG019.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG019.json -h SekiuWA_FG019.html 
fastp v0.20.0, time used: 28 seconds