WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... ACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAA Read1 before filtering: total reads: 1424295 total bases: 205098480 Q20 bases: 198688095(96.8745%) Q30 bases: 190483357(92.8741%) Read1 after filtering: total reads: 1402795 total bases: 201953017 Q20 bases: 196020609(97.0625%) Q30 bases: 188001742(93.0918%) Filtering result: reads passed filter: 1402795 reads failed due to low quality: 4097 reads failed due to too many N: 1826 reads failed due to too short: 15577 reads with adapter trimmed: 22017 bases trimmed due to adapters: 2290892 Duplication rate (may be overestimated since this is SE data): 76.4303% JSON report: SekiuWA_FG018.json HTML report: SekiuWA_FG018.html fastp -i SekiuWA_FG018.F.fq.gz -o SekiuWA_FG018.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG018.json -h SekiuWA_FG018.html fastp v0.20.0, time used: 17 seconds