WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAA

Read1 before filtering:
total reads: 2332687
total bases: 335906928
Q20 bases: 325494828(96.9003%)
Q30 bases: 311620403(92.7699%)

Read1 after filtering:
total reads: 2305350
total bases: 331925540
Q20 bases: 322303058(97.101%)
Q30 bases: 308708718(93.0054%)

Filtering result:
reads passed filter: 2305350
reads failed due to low quality: 6994
reads failed due to too many N: 3211
reads failed due to too short: 17132
reads with adapter trimmed: 24065
bases trimmed due to adapters: 2509736

Duplication rate (may be overestimated since this is SE data): 68.5194%

JSON report: SekiuWA_FG016.json
HTML report: SekiuWA_FG016.html

fastp -i SekiuWA_FG016.F.fq.gz -o SekiuWA_FG016.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG016.json -h SekiuWA_FG016.html 
fastp v0.20.0, time used: 22 seconds