WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... TTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACCATTT Read1 before filtering: total reads: 1774998 total bases: 255599712 Q20 bases: 248267073(97.1312%) Q30 bases: 238325207(93.2416%) Read1 after filtering: total reads: 1758955 total bases: 253258809 Q20 bases: 246453866(97.313%) Q30 bases: 236676220(93.4523%) Filtering result: reads passed filter: 1758955 reads failed due to low quality: 4574 reads failed due to too many N: 2404 reads failed due to too short: 9065 reads with adapter trimmed: 13941 bases trimmed due to adapters: 1334453 Duplication rate (may be overestimated since this is SE data): 71.8172% JSON report: SekiuWA_FG011.json HTML report: SekiuWA_FG011.html fastp -i SekiuWA_FG011.F.fq.gz -o SekiuWA_FG011.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG011.json -h SekiuWA_FG011.html fastp v0.20.0, time used: 18 seconds