WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... CAAAAGCAATATCAGCACCAACAGAAACAACCTGATTAGCGGCGTTGACAGATGTATCCA Read1 before filtering: total reads: 2851503 total bases: 410616432 Q20 bases: 398727714(97.1047%) Q30 bases: 382672722(93.1947%) Read1 after filtering: total reads: 2826495 total bases: 406944993 Q20 bases: 395927768(97.2927%) Q30 bases: 380138291(93.4127%) Filtering result: reads passed filter: 2826495 reads failed due to low quality: 7705 reads failed due to too many N: 3922 reads failed due to too short: 13381 reads with adapter trimmed: 22865 bases trimmed due to adapters: 1994878 Duplication rate (may be overestimated since this is SE data): 64.7932% JSON report: SekiuWA_FG006.json HTML report: SekiuWA_FG006.html fastp -i SekiuWA_FG006.F.fq.gz -o SekiuWA_FG006.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG006.json -h SekiuWA_FG006.html fastp v0.20.0, time used: 32 seconds