WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... CCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCTGGAGACAAATAATCTCTT Read1 before filtering: total reads: 3147835 total bases: 453288240 Q20 bases: 440045283(97.0785%) Q30 bases: 421938543(93.0839%) Read1 after filtering: total reads: 3122068 total bases: 449549680 Q20 bases: 437236815(97.2611%) Q30 bases: 419409101(93.2954%) Filtering result: reads passed filter: 3122068 reads failed due to low quality: 8155 reads failed due to too many N: 4248 reads failed due to too short: 13364 reads with adapter trimmed: 16664 bases trimmed due to adapters: 1949619 Duplication rate (may be overestimated since this is SE data): 63.1384% JSON report: SekiuWA_FG005.json HTML report: SekiuWA_FG005.html fastp -i SekiuWA_FG005.F.fq.gz -o SekiuWA_FG005.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_FG005.json -h SekiuWA_FG005.html fastp v0.20.0, time used: 31 seconds