WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4067122
total bases: 585665568
Q20 bases: 566835265(96.7848%)
Q30 bases: 532130232(90.8591%)

Read1 after filtering:
total reads: 4052874
total bases: 583583253
Q20 bases: 565606285(96.9196%)
Q30 bases: 531246622(91.0318%)

Filtering result:
reads passed filter: 4052874
reads failed due to low quality: 8390
reads failed due to too many N: 5858
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.4385%

JSON report: SekiuWA_67.json
HTML report: SekiuWA_67.html

fastp -i SekiuWA_67.F.fq.gz -o SekiuWA_67.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_67.json -h SekiuWA_67.html 
fastp v0.20.0, time used: 47 seconds