WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4224276
total bases: 608295744
Q20 bases: 589911988(96.9778%)
Q30 bases: 555006581(91.2396%)

Read1 after filtering:
total reads: 4210352
total bases: 606259573
Q20 bases: 588688019(97.1016%)
Q30 bases: 554105535(91.3974%)

Filtering result:
reads passed filter: 4210352
reads failed due to low quality: 7755
reads failed due to too many N: 6169
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 57.7875%

JSON report: SekiuWA_66.json
HTML report: SekiuWA_66.html

fastp -i SekiuWA_66.F.fq.gz -o SekiuWA_66.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_66.json -h SekiuWA_66.html 
fastp v0.20.0, time used: 49 seconds