WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2320119
total bases: 334097136
Q20 bases: 323546683(96.8421%)
Q30 bases: 304155002(91.0379%)

Read1 after filtering:
total reads: 2312517
total bases: 332943296
Q20 bases: 322879778(96.9774%)
Q30 bases: 303677093(91.2099%)

Filtering result:
reads passed filter: 2312517
reads failed due to low quality: 4381
reads failed due to too many N: 3221
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 58.5642%

JSON report: SekiuWA_65.json
HTML report: SekiuWA_65.html

fastp -i SekiuWA_65.F.fq.gz -o SekiuWA_65.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_65.json -h SekiuWA_65.html 
fastp v0.20.0, time used: 30 seconds