WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1985823
total bases: 285958512
Q20 bases: 276614663(96.7324%)
Q30 bases: 259802850(90.8533%)

Read1 after filtering:
total reads: 1978615
total bases: 284905066
Q20 bases: 276004628(96.876%)
Q30 bases: 259368257(91.0367%)

Filtering result:
reads passed filter: 1978615
reads failed due to low quality: 4452
reads failed due to too many N: 2756
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 62.7182%

JSON report: SekiuWA_63.json
HTML report: SekiuWA_63.html

fastp -i SekiuWA_63.F.fq.gz -o SekiuWA_63.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_63.json -h SekiuWA_63.html 
fastp v0.20.0, time used: 28 seconds