WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2823636
total bases: 406603584
Q20 bases: 394342825(96.9846%)
Q30 bases: 371198009(91.2924%)

Read1 after filtering:
total reads: 2813828
total bases: 405153080
Q20 bases: 393492419(97.1219%)
Q30 bases: 370584986(91.4679%)

Filtering result:
reads passed filter: 2813828
reads failed due to low quality: 5748
reads failed due to too many N: 4060
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 63.0316%

JSON report: SekiuWA_62.json
HTML report: SekiuWA_62.html

fastp -i SekiuWA_62.F.fq.gz -o SekiuWA_62.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_62.json -h SekiuWA_62.html 
fastp v0.20.0, time used: 36 seconds