WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3530122
total bases: 508337568
Q20 bases: 492833269(96.95%)
Q30 bases: 464178353(91.313%)

Read1 after filtering:
total reads: 3517600
total bases: 506483214
Q20 bases: 491750437(97.0912%)
Q30 bases: 463397849(91.4932%)

Filtering result:
reads passed filter: 3517600
reads failed due to low quality: 7391
reads failed due to too many N: 5131
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 63.5056%

JSON report: SekiuWA_60.json
HTML report: SekiuWA_60.html

fastp -i SekiuWA_60.F.fq.gz -o SekiuWA_60.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_60.json -h SekiuWA_60.html 
fastp v0.20.0, time used: 43 seconds