WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2248611
total bases: 323799984
Q20 bases: 313770439(96.9025%)
Q30 bases: 295198893(91.167%)

Read1 after filtering:
total reads: 2240125
total bases: 322563080
Q20 bases: 313043899(97.0489%)
Q30 bases: 294677448(91.355%)

Filtering result:
reads passed filter: 2240125
reads failed due to low quality: 5095
reads failed due to too many N: 3391
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 58.6227%

JSON report: SekiuWA_51.json
HTML report: SekiuWA_51.html

fastp -i SekiuWA_51.F.fq.gz -o SekiuWA_51.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_51.json -h SekiuWA_51.html 
fastp v0.20.0, time used: 30 seconds