WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1820301
total bases: 262123344
Q20 bases: 253787978(96.8201%)
Q30 bases: 238291324(90.9081%)

Read1 after filtering:
total reads: 1814259
total bases: 261235578
Q20 bases: 253265235(96.949%)
Q30 bases: 237914594(91.0728%)

Filtering result:
reads passed filter: 1814259
reads failed due to low quality: 3523
reads failed due to too many N: 2519
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 57.8868%

JSON report: SekiuWA_50.json
HTML report: SekiuWA_50.html

fastp -i SekiuWA_50.F.fq.gz -o SekiuWA_50.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_50.json -h SekiuWA_50.html 
fastp v0.20.0, time used: 25 seconds