WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 5237294
total bases: 754170336
Q20 bases: 730665117(96.8833%)
Q30 bases: 685941498(90.9531%)

Read1 after filtering:
total reads: 5232980
total bases: 753328544
Q20 bases: 730251079(96.9366%)
Q30 bases: 685672639(91.0191%)

Filtering result:
reads passed filter: 5232980
reads failed due to low quality: 2644
reads failed due to too many N: 1670
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 46.1488%

JSON report: SekiuWA_49.json
HTML report: SekiuWA_49.html

fastp -i SekiuWA_49.F.fq.gz -o SekiuWA_49.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_49.json -h SekiuWA_49.html 
fastp v0.20.0, time used: 60 seconds