WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2798645
total bases: 403004880
Q20 bases: 390746589(96.9583%)
Q30 bases: 367434920(91.1738%)

Read1 after filtering:
total reads: 2789402
total bases: 401646531
Q20 bases: 389945788(97.0868%)
Q30 bases: 366856802(91.3382%)

Filtering result:
reads passed filter: 2789402
reads failed due to low quality: 5406
reads failed due to too many N: 3837
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 54.3742%

JSON report: SekiuWA_46.json
HTML report: SekiuWA_46.html

fastp -i SekiuWA_46.F.fq.gz -o SekiuWA_46.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_46.json -h SekiuWA_46.html 
fastp v0.20.0, time used: 35 seconds