WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3165976
total bases: 455900544
Q20 bases: 441817663(96.911%)
Q30 bases: 415711982(91.1848%)

Read1 after filtering:
total reads: 3155204
total bases: 454294007
Q20 bases: 440882037(97.0477%)
Q30 bases: 415038949(91.3591%)

Filtering result:
reads passed filter: 3155204
reads failed due to low quality: 6265
reads failed due to too many N: 4507
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 53.8257%

JSON report: SekiuWA_45.json
HTML report: SekiuWA_45.html

fastp -i SekiuWA_45.F.fq.gz -o SekiuWA_45.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_45.json -h SekiuWA_45.html 
fastp v0.20.0, time used: 39 seconds