WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2763164
total bases: 397895616
Q20 bases: 385769224(96.9524%)
Q30 bases: 363228647(91.2874%)

Read1 after filtering:
total reads: 2754052
total bases: 396547181
Q20 bases: 384967837(97.08%)
Q30 bases: 362643218(91.4502%)

Filtering result:
reads passed filter: 2754052
reads failed due to low quality: 5127
reads failed due to too many N: 3985
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 57.1276%

JSON report: SekiuWA_44.json
HTML report: SekiuWA_44.html

fastp -i SekiuWA_44.F.fq.gz -o SekiuWA_44.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_44.json -h SekiuWA_44.html 
fastp v0.20.0, time used: 36 seconds