WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3858880
total bases: 555678720
Q20 bases: 537224725(96.679%)
Q30 bases: 504484809(90.7871%)

Read1 after filtering:
total reads: 3845341
total bases: 553672942
Q20 bases: 536060778(96.819%)
Q30 bases: 503653367(90.9659%)

Filtering result:
reads passed filter: 3845341
reads failed due to low quality: 8064
reads failed due to too many N: 5475
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 58.6954%

JSON report: SekiuWA_41.json
HTML report: SekiuWA_41.html

fastp -i SekiuWA_41.F.fq.gz -o SekiuWA_41.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_41.json -h SekiuWA_41.html 
fastp v0.20.0, time used: 46 seconds