WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3135082
total bases: 451451808
Q20 bases: 436913426(96.7796%)
Q30 bases: 410773310(90.9894%)

Read1 after filtering:
total reads: 3124042
total bases: 449820870
Q20 bases: 435969911(96.9208%)
Q30 bases: 410099869(91.1696%)

Filtering result:
reads passed filter: 3124042
reads failed due to low quality: 6643
reads failed due to too many N: 4397
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 56.4813%

JSON report: SekiuWA_39.json
HTML report: SekiuWA_39.html

fastp -i SekiuWA_39.F.fq.gz -o SekiuWA_39.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SekiuWA_39.json -h SekiuWA_39.html 
fastp v0.20.0, time used: 39 seconds