WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4242094
total bases: 610861536
Q20 bases: 592444518(96.9851%)
Q30 bases: 559108387(91.5278%)

Read1 after filtering:
total reads: 4225709
total bases: 608472914
Q20 bases: 591065858(97.1392%)
Q30 bases: 558124843(91.7255%)

Filtering result:
reads passed filter: 4225709
reads failed due to low quality: 10222
reads failed due to too many N: 6163
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 63.9199%

JSON report: PortGambleWA_FG196.json
HTML report: PortGambleWA_FG196.html

fastp -i PortGambleWA_FG196.F.fq.gz -o PortGambleWA_FG196.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG196.json -h PortGambleWA_FG196.html 
fastp v0.20.0, time used: 48 seconds