WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4125328
total bases: 594047232
Q20 bases: 575423631(96.865%)
Q30 bases: 541940128(91.2285%)

Read1 after filtering:
total reads: 4110462
total bases: 591847379
Q20 bases: 574160264(97.0115%)
Q30 bases: 541040293(91.4155%)

Filtering result:
reads passed filter: 4110462
reads failed due to low quality: 9053
reads failed due to too many N: 5813
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 64.9484%

JSON report: PortGambleWA_FG195.json
HTML report: PortGambleWA_FG195.html

fastp -i PortGambleWA_FG195.F.fq.gz -o PortGambleWA_FG195.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG195.json -h PortGambleWA_FG195.html 
fastp v0.20.0, time used: 49 seconds