WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2620007
total bases: 377281008
Q20 bases: 366184549(97.0588%)
Q30 bases: 345033428(91.4526%)

Read1 after filtering:
total reads: 2610780
total bases: 375935390
Q20 bases: 365394296(97.196%)
Q30 bases: 344462925(91.6282%)

Filtering result:
reads passed filter: 2610780
reads failed due to low quality: 5423
reads failed due to too many N: 3804
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 68.6057%

JSON report: PortGambleWA_FG194.json
HTML report: PortGambleWA_FG194.html

fastp -i PortGambleWA_FG194.F.fq.gz -o PortGambleWA_FG194.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG194.json -h PortGambleWA_FG194.html 
fastp v0.20.0, time used: 33 seconds