WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3182779
total bases: 458320176
Q20 bases: 444276723(96.9359%)
Q30 bases: 418554371(91.3236%)

Read1 after filtering:
total reads: 3170636
total bases: 456534225
Q20 bases: 443253444(97.091%)
Q30 bases: 417829929(91.5221%)

Filtering result:
reads passed filter: 3170636
reads failed due to low quality: 7523
reads failed due to too many N: 4620
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 67.397%

JSON report: PortGambleWA_FG193.json
HTML report: PortGambleWA_FG193.html

fastp -i PortGambleWA_FG193.F.fq.gz -o PortGambleWA_FG193.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG193.json -h PortGambleWA_FG193.html 
fastp v0.20.0, time used: 39 seconds