WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 5157010
total bases: 742609440
Q20 bases: 719842002(96.9341%)
Q30 bases: 677249579(91.1986%)

Read1 after filtering:
total reads: 5137830
total bases: 739794527
Q20 bases: 718198418(97.0808%)
Q30 bases: 676069347(91.3861%)

Filtering result:
reads passed filter: 5137830
reads failed due to low quality: 11545
reads failed due to too many N: 7635
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 71.7563%

JSON report: PortGambleWA_FG192.json
HTML report: PortGambleWA_FG192.html

fastp -i PortGambleWA_FG192.F.fq.gz -o PortGambleWA_FG192.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG192.json -h PortGambleWA_FG192.html 
fastp v0.20.0, time used: 58 seconds