WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 9667622 total bases: 1392137568 Q20 bases: 1345051693(96.6177%) Q30 bases: 1265776455(90.9232%) Read1 after filtering: total reads: 9633917 total bases: 1387192234 Q20 bases: 1342146426(96.7527%) Q30 bases: 1263677406(91.0961%) Filtering result: reads passed filter: 9633917 reads failed due to low quality: 19863 reads failed due to too many N: 13842 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 66.7226% JSON report: PortGambleWA_FG191.json HTML report: PortGambleWA_FG191.html fastp -i PortGambleWA_FG191.F.fq.gz -o PortGambleWA_FG191.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG191.json -h PortGambleWA_FG191.html fastp v0.20.0, time used: 100 seconds