WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4741206
total bases: 682733664
Q20 bases: 660743232(96.7791%)
Q30 bases: 620630941(90.9038%)

Read1 after filtering:
total reads: 4724707
total bases: 680299586
Q20 bases: 659313725(96.9152%)
Q30 bases: 619601544(91.0777%)

Filtering result:
reads passed filter: 4724707
reads failed due to low quality: 9626
reads failed due to too many N: 6873
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 63.582%

JSON report: PortGambleWA_FG188.json
HTML report: PortGambleWA_FG188.html

fastp -i PortGambleWA_FG188.F.fq.gz -o PortGambleWA_FG188.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG188.json -h PortGambleWA_FG188.html 
fastp v0.20.0, time used: 53 seconds