WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 6240268 total bases: 898598592 Q20 bases: 870516677(96.8749%) Q30 bases: 818235039(91.0568%) Read1 after filtering: total reads: 6218413 total bases: 895397156 Q20 bases: 868627652(97.0103%) Q30 bases: 816871328(91.2301%) Filtering result: reads passed filter: 6218413 reads failed due to low quality: 12800 reads failed due to too many N: 9055 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 60.85% JSON report: PortGambleWA_FG187.json HTML report: PortGambleWA_FG187.html fastp -i PortGambleWA_FG187.F.fq.gz -o PortGambleWA_FG187.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG187.json -h PortGambleWA_FG187.html fastp v0.20.0, time used: 65 seconds