WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3878840
total bases: 558552960
Q20 bases: 541460587(96.9399%)
Q30 bases: 509575815(91.2314%)

Read1 after filtering:
total reads: 3865664
total bases: 556622442
Q20 bases: 540308062(97.069%)
Q30 bases: 508733160(91.3965%)

Filtering result:
reads passed filter: 3865664
reads failed due to low quality: 7452
reads failed due to too many N: 5724
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 61.9498%

JSON report: PortGambleWA_FG186.json
HTML report: PortGambleWA_FG186.html

fastp -i PortGambleWA_FG186.F.fq.gz -o PortGambleWA_FG186.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG186.json -h PortGambleWA_FG186.html 
fastp v0.20.0, time used: 46 seconds