WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2843361
total bases: 409443984
Q20 bases: 395068948(96.4891%)
Q30 bases: 370694373(90.536%)

Read1 after filtering:
total reads: 2822341
total bases: 406382523
Q20 bases: 393647274(96.8662%)
Q30 bases: 369814803(91.0017%)

Filtering result:
reads passed filter: 2822341
reads failed due to low quality: 16949
reads failed due to too many N: 4071
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 63.5076%

JSON report: PortGambleWA_FG185.json
HTML report: PortGambleWA_FG185.html

fastp -i PortGambleWA_FG185.F.fq.gz -o PortGambleWA_FG185.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG185.json -h PortGambleWA_FG185.html 
fastp v0.20.0, time used: 35 seconds